FIG 3.

Gene expression analysis of ecnAB in the rpfF mutant strain. Relative expression of the ecnA, ecnB, and gumB genes in the rpfF mutant compared to the wild-type strain. gumB was used as a control that is positively regulated by RpfF. Transcript abundance was determined by real-time RT-qPCR. Data are shown as the mean of three independent biological replicates, and error bars indicate the standard deviation of the mean. 16S rRNA was used as an endogenous control. *, significant difference (P < 0.05); ns, no significant difference.