FIG 4.

Biofilm formation by the X. citri wild-type, ecnA::Tn5, ΔecnA, ecnA::Tn5_c, and ΔrpfF strains. Biofilms formed on abiotic (A) and biotic (C) surfaces were stained with crystal violet and quantified at an OD of 590 nm (B and D). The values in panel B were normalized to bacterial growth (OD at 600 nm). The picture of leaves is representative of six independent replicates. Error bars indicate the standard deviation of five replicates. *, significant difference (P < 0.05) compared with the wild-type strain.