FIG 6.

Effect of wild-type (wt), ecnA::Tn5, complemented (ecnA::Tn5_c), and ΔecnA strains on sliding motility. (A) Diameters of cell sliding on plates. *, significant difference (P < 0.05); ns, no significant difference. Error bars indicate the standard deviation of the mean. (B) ecnA::Tn5 and ΔecnA strains showed a decrease in motility that could be restored to wild-type levels by the introduction of a plasmid containing the intact ecnA gene. Cells from bacterial cultures at the exponential stage were spotted on SB plates supplemented with 0.5% agar. The plates were incubated at 28°C and photographed after 2 days.