FIG 7.

Bacterial growth and pathogenicity assay in planta. (A) Populations of wild-type and ecnA::Tn5 bacteria inoculated by infiltration at a concentration of 10⁸ CFU/ml and analyzed at 7, 14, and 21 days after inoculation (dai). (B) Symptoms of sweet orange leaves infiltrated with the wild type and the ecnA::Tn5 strain at 21 dai. (C) Symptoms of sweet orange detached leaves infiltrated with wild-type, ecnA::Tn5, ΔecnA, and ecnA::Tn5_c strains at 14 dai. (D) Populations of the wild type and ecnA::Tn5 mutant bacteria inoculated by spraying at a concentration of 10⁸ CFU/ml and analyzed at 7, 14, and 21 dai. (E) Severity of citrus canker symptoms at 21 dai. Error bars indicate the standard deviation of the mean of two independent experiments. *, significant difference (P < 0.05). Images are representative of five independent replicates.