FIG 6.

Pilin levels are unaffected by altering levels of σ^H or σ^E. Western blot analysis of pilin levels in whole-cell lysates collected from bacteria lacking σ^E (S4 ΔrpoE) or with inducible expression of σ^H or σ^E (S4 P_lac-rpoH and S4 P_lac-rpoE), or from a control strain (S4 ery), on solid medium at 37°C is shown. Expression of IPTG-induced σ^E and σ^H was detected using anti-FLAG antibody. No change in pilin levels was observed when σ factors were deleted or induced. RecA was used as a control, and the blot is a representative from two independent experiments. Numbers to the leftls indicate the positions of molecular weight markers (kilodaltons).