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(a and b) Dose-response curves for the activation of the lux-based agrP3 reporter via AgrC1 and AgrC1 N267I mutant (a) or AgrC1 Y251F mutant (b), respectively. (c) Bioluminescent agrP3-lux reporter expression profiles for strains MY15 and MY20 carrying AgrC1 or AgrC1 N267I, respectively, with or without the addition of AIP-1 or (Ala5)AIP-1 at the time of inoculation. All assays were performed in triplicate; error bars represent the SEM. OD, optical density; RLU, relative light units.
