(a) Growth and AIP production for agr group 1 strains ICP5014 (WT), ICP5062 (T247I), IU12 (I311T), and HU24 (I311T/A343T). (b) Dose-response curves for the AIP-1-dependent activation of the lux-based agrP3 reporters incorporating AgrC1 A343T, AgrC1 I311T, or AgrC1 I311T-A343T. (c) Dose-response curves for the AIP-1-dependent activation of lux-based agrP3 reporters incorporating AgrC1 L245A, F264A, and T247I, respectively. All assays were performed in triplicate; error bars represent the SEM. Hashed lines indicate the growth curves. OD, optical density; RLU, relative light units.