FIG 2.

SpSL1 phage relative gene expression in lytic and lysogenic stages. (A to C) A time course of lytic infection of an spnIII-deleted strain at an MOI of 0.2 at 10 min (A) (green), 50 min (B) (blue), and 90 min (C) (red) after challenge. (D) Gene expression in the lysogen (black). All data are shown in terms of the normalized read coverage. (E) RNA sequencing reads were mapped to the viral conformation of the SpSL1 phage deposited in GenBank (accession number KM882824), even in the case of the lysogen (D). RNA-seq mapping and upper quartile normalization were performed using Rockhopper software. Data were visualized on BAMviewer in the Artemis tool, with the maximum number of reads being 2,000 (small label on the right of each panel). RNA-seq data were deposited at Gene Expression Omnibus GEO (accession number GSE132611).