FIG 4.

S. pneumoniae genes are upregulated in response to SpSL1 infection. RNA-seq analysis during SpSL1 infection revealed the upregulation of three S. pneumoniae transcripts preceded by an NrdR binding site and encoding the products of the anaerobic ribonucleoside triphosphate reductase operon (circles; SPD_0187 to SPD_0191, a five-gene operon), the ribonucleoside diphosphate reductase operon (squares; SPD_1041 to SPD_1043, a three-gene operon), and a hypothetical operon encoding an unknown transcriptional regulator and a conserved hypothetical protein (triangles; SPD_1594 and SPD_1595, a two-gene operon). The RNA-seq data were normalized by upper quartile gene normalization and compared with those for a noninfected control to determine the fold change in expression.