TABLE 3.

Primers used in this study

Primer name	Sequence (5′ → 3′)
Deletion alleles
Δhcp
Δhcp-5-KpnI-u	GGGGTACCTCCAGTTTAACTAATTGGAATCGAC
Δhcp-5-XbaI-l	GGTCTAGACATATATGCTGGAGTTGGCATGCT
Δhcp-3-XbaI-u	GGTCTAGAACACCACGCGAAGCATAATCGTTT
Δhcp-3-SacI-l	GGGAGCTCTTATCAGTATAAATTTCTTCACC
Δhcp1
Δhcp1-5-KpnI-u	GGGGTACCGTCGGTTTGGGTATTCCGGGAATG
Δhcp1-5-XbaI-l	GGTCTAGACATATACGCTGGAGTTGGCATGGC
Δhcp1-3-XbaI-u	GGTCTAGAACACCGCGCGAAGCGTAATGATTA
Δhcp1-3-SacI-l	GGGAGCTCGTCACTAGAGGTCTGTCTGGGTCC
Complementation vectors
hcp+
hcp-XbaI-u	GCTCTAGAAGTTAGTTTCAATCATTAGCCAGAG
hcp-KpnI-l	GTGGTACCATCTTTTACCTGATACTAATATGCA
hcp1+
hcp1-XbaI-u	GCTCTAGACTTGGAGCCTTATAATTTTAGAACAC
hcp1-KpnI-l	GTGGTACCTCATAAACCATAAGCAATGCAATAG
IPTG-inducible vectors
Ptrc-hcp-u	CACGTCGACGTTGACACTCTATCATTGATAG
Ptrc-hcp-l	GAAGATTTTCTTATTTGTATAGTTCATCC