FIG 6.
Susceptibility to antibiotics and altered cellular permeability in the ΔatpD strain. (A and B) Growth profiles of the WT, ΔatpD, and ΔatpDC strains in the presence of 20 μg/ml of isoniazid (INH) and 30 μg/ml of triclosan, respectively. The data were recorded every 3 h after antibiotic addition at time zero. Reduced growth was observed for the ΔatpD strain compared to the other two. (C and D) Uptake of Nile red dye and ethidium bromide, respectively. In both cases, the knockout strain showed higher fluorescence due to increased uptake. In each panel, data are representative of those from three independent experiments, with error bars denoting standard deviations. *, P ≤ 0.05; **, P ≤ 0.01. RFU, relative fluorescence units.