FIG 4.

Mechanism of PinT regulation of hilA or rtsA mRNA translation. Shown is β-galactosidase activity in Salmonella strains containing the hilA′-′lacZ translational fusion (A and B), rtsA′-′lacZ translational fusion (C), or rtsB′-lacZ⁺ transcriptional fusion (D) in the indicated genetic backgrounds with either the empty vector or plasmids overexpressing PinT. β-Galactosidase activity units are defined as (μmol of ONP formed min⁻¹) × 10⁶/(OD₆₀₀ × ml of cell suspension) and are reported as mean ± standard deviation, where n = 3. P values (unpaired t test) are indicated as follows: ***, P < 0.001. The strains used are JS2333, JS2337, JS2336, JS2338, JS2334, JS2336, JS325, and JS2339, each with plasmid pBRplac or pPinT.