Figure 6.

Validation of RNA-Seq analysis by qPCR. a, selected up-regulated pathways and genes (with fold-change (log2) and p value) from RNA-Seq analysis. Verification of b, cell-cell/cell-ECM signaling pathway; c, EGFR signaling pathway; and d, Rho-GTPase signaling pathway was performed by qPCR. Data are plotted as the relative fold-change increase in genes of P152Lp53 tumor over pCMV10 vector control tumor (n = 2). Individual fold-change values are indicated above the bar. All error bars are calculated using standard deviation.