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Competitive TR-FRET identifies LIR peptides with differential binding to mATG8 proteins. Unlabeled LIR peptides were used to compete FITC-p62 binding to mATG8 proteins for FYCO1 (A), ULK1 (B), ALFY (C), TP53INP2 (D), and TP53INP1 (E). The data are representative of three independent experiments. The error bars represent standard deviation from two technical replicates.
