Figure 5.

The C-terminal cysteine of the TP53INP1 peptide interacts with a nonconserved loop region in Atg8 but not LC3B. A and E, overlays of 2D ¹⁵N-¹H HSQC spectra of ¹⁵N-labeled Atg8 (A) and LC3B (E) titrated with peptide 2 (blue), peptide 8 (red), and peptide 6 (green). B, C, F, and G, select perturbed residues are circled and mapped in magenta on the structural model of Atg8 (B, Protein Data Bank code 2LI5) in complex with Atg7C30 (cyan in C) and LC3B (F, Protein Data Bank code 5WRD) in complex with FYCO1 LIR (cyan in G), respectively. C and G, the distance between the side chain C^δ from Leu-55 (magenta, C) of Atg8 and the C^β from Ala (red, C) of Atg7C30 and between the side chain N^ϵ from His-57 (magenta, G) of LC3B and the S^γ from Cys (red, G) of FYCO1 LIR are indicated. D, sequence alignment for the nonconserved DLTV regions for Atg8 and mATG8.