Figure 3.

α₂M*/CS-GRP78 axis regulates Rho signaling to activate YAP and TAZ. A, PDAC cell lines stimulated with α₂M* (100 pm) for 30 min in the absence or presence of Scr peptide (100 pm), GRP78 peptide (100 pm), or C38 mAb (50 μg) for 6 h or fasudil (5 μm) for 16 h and probed for indicated proteins. B, RhoA-silenced PDAC cell lines stimulated with α₂M* (100 pm) for 30 min in the absence or presence of Scr peptide (100 pm) or GRP78 peptide (100 pm) for 6 h or fasudil (5 μm) for 16 h and probed for indicated proteins. C, PDAC cell lines were transduced with Rho-T19N and then stimulated with α₂M* (100 pm) for 30 min in the absence or presence of Scr peptide (100 pm) or GRP78 peptide (100 pm) for 6 h or fasudil (5 μm) for 16 h and probed for indicated proteins. D, cytoplasmic and nuclear localization of YAP/TAZ were analyzed in the indicated cancer cell lines stimulated with α₂M* (100 pm) for 30 min in the absence or presence of Scr peptide (100 pm), GRP78 peptide (100 pm), or C38 mAb (50 μg) for 6 h or fasudil (5 μm) for 16 h. E, cytoplasmic and nuclear localization of YAP/TAZ were analyzed in the RhoA-silenced PDAC cell lines stimulated with α₂M* (100 pm) for 30 min in the absence or presence of Scr peptide (100 pm), GRP78 peptide (100 pm), or C38 mAb (50 μg) for 6 h or fasudil (5 μm) for 16 h. UT, untransfected.