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. 2019 Jul 29;294(38):13939–13952. doi: 10.1074/jbc.RA119.009091

Figure 4.

Figure 4.

YAP/TAZ signaling promotes PDAC cell motility and invasion by α2M*/CS-GRP78 axis. A, immunoblot analysis of indicated proteins in YAP/TAZ-silenced PDAC cell lines were stimulated with α2M* (100 pm) for 30 min in the absence or presence of Scr peptide (100 pm) or GRP78 peptide (100 pm) for 6 h or fasudil (5 μm) for 16 h. B, Rho activation assay was measured in YAP/TAZ-silenced PDAC cell lines, stimulated with α2M* (100 pm) for 30 min in the absence or presence of Scr peptide (100 pm), GRP78 peptide (100 pm), or C38 mAb (50 μg) for 6 h or fasudil (5 μm) for 16 h. C, YAP/TAZ-silenced PDAC cell lines were stimulated with α2M* (100 pm) for 30 min in the absence or presence of Scr peptide (100 pm), GRP78 peptide (100 pm), or C38 mAb (50 μg) for 6 h or fasudil (5 μm) for 16 h to analyze migration and invasion by EZCellTM cell migration and Matrigel invasion assay. Mean ± S.D. of triplicates is shown. D, quantitative RT-PCR analysis was performed in PDAC cell lines stimulated with α2M* (100 pm) for 30 min in the absence or presence of Scr peptide (100 pm), GRP78 peptide (100 pm), or C38 mAb (50 μg) for 6 h or fasudil (5 μm) for 16 h to quantify the transcript levels of the YAP/TAZ target genes Ctgf, Cyr61, and Axl.