Figure 3.
In vitro time-kill activities of (A) 5.2d, (B) 5.2e, (C) 5.6a, (D) 5.8 and (E) 5.8a. Time-kill activity was determined using an in vitro time-kill kinetics assay in which the compounds were co-incubated with C. neoformans cells at 37°C, 5% CO2, pH 7.4. The number of CFU is counted during 96 h of incubation. All of the compounds displayed antifungal activity in a dose-dependent manner.