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. Author manuscript; available in PMC: 2019 Sep 23.
Published in final edited form as: J Vis Exp. 2019 Jul 26;(149):10.3791/59894. doi: 10.3791/59894

Figure 2. Relative expression of stem cell and differentiation markers of human ileal enteroids grown in ECM or differentiated as monolayers.

Figure 2.

Ileal enteroids were grown for 7 days in ECM (Undifferentiated) or grown and differentiated as monolayers without (Differentiated) or with RANKL and TNFα (Differentiated +R/T). Ileal enteroid cultures or monolayers were harvested in Trizol for RNA extraction. Gene expression was determined by qRT-PCR and is expressed relative to GAPDH. Data is average of 3 independent wells of ileal enteroids or monolayers per condition. Error bars indicate SEM. ND is not detected. Statistical significance was determined on log-transformed values using one-way ANOVA with Dunnett’s multiple comparisons test comparing to the Undifferentiated. ** p<0.01, *** p<0.001