Fig. 3.

BCR/ABL-induced tumor formation in vivo is impaired upon loss of STAT5B. a Left: Subcutaneous injection of BCR/ABL^p185+ established cell lines into NSG mice (n = 8 per genotype). Bar graph summarizes tumor weights. Levels of significance were calculated using one-way ANOVA. Error bars represent mean ± SEM. Right: Representative pictures of tumor sizes are depicted. b Kaplan–Meier plot of NSG mice that have received an intravenous injection (i.v.) of wt, Stat5a^−/−, or Stat5b^−/− BCR/ABL^p185+ cells (n ≥ 6 per genotype). Log-rank (Mantel–Cox) test was used to calculate levels of significance. Right: I.v. injection of wt, Stat5a^−/−, or Stat5b^−/− BCR/ABL^p185+ cells into NSG mice. Three mice per genotype were sacrificed on day 5, day 8, and day 11 upon injection of BCR/ABL^p185+ cells. Quantitative analysis via FACS for GFP⁺ BCR/ABL^p185+ cells in the BM (n = 3 per genotype and day) as well as representative histograms of each day and phenotype are provided. Error bars represent mean ± SEM. Levels of significance were calculated using Kruskal–Wallis test followed by Dunn’s test. c Survival plots after new-born infections using Ab-MulV (encoding v-abl) comparing left: wt, Stat5a^+/−, and Stat5a^−/− mice (n ≥ 3 per genotype) or right: wt, Stat5b^+/−, and Stat5b^−/− mice (n ≥ 4 per genotype). Log-rank (Mantel–Cox) test was used to calculate levels of significance. NSG NOD.Cg-Prkdc^scid Il2rgtm ^1Wjl/SzJ, ANOVA analysis of variance, IFN interferon, STAT signal transducers and activators of transcription, wt wild type, GFP green fluorescent protein