Fig. 4.

mTORC1 and mTORC2 physically associate with p53 during PICS. a MCF7 cells were transfected with vectors encoding Flag-tagged wild-type (WT) mTOR or Flag-tagged kinase-dead (KD) mTOR. The next day, the cells were transfected with the indicated siRNAs. Two days later, WB analyses were conducted. b, d MCF7 cells were transfected with Con Si, PTEN Si (b, c), or Myr-AKT (d). After 2 days, the transfected cells were lysed, immunoprecipitated with α-p53, α-Raptor, and α-Rictor antibodies, and immunoblotted with the indicated antibodies. e In vitro competition assay. f MCF7 cells were transfected with Con Si or mTOR Si. After 6 h, half of each group was transfected with Con Si, and the other half was transfected with PTEN Si. After 2 days, transfected cells were lysed and immunoprecipitated with anti-p53 antibody