Fig. 5.

mTORC1 and mTORC2 directly phosphorylate p53 at S15 in PICS. a MCF7 cells exogenously expressing flag-tagged WT, Δ40, and Δ133 p53 along with Myc-tagged mTOR were transfected with Con Si or PTEN Si. Two days after siRNA transfection, the cells were immunoprecipitated with anti-Flag antibody. b Cells were transfected with Con Si or PTEN Si, harvested at the indicated days, and subjected to immunoblotting. c In vitro kinase assay. MCF7 cells were transfected with Con Si or PTEN Si. After 2 days, cell lysates were immunoprecipitated with α-Raptor and α-Rictor antibodies and used for in vitro kinase assay. d MCF7 cells exogenously expressing Flag-tagged WT p53 or S15A mutant p53, in combination with Myc-tagged mTOR, were transfected with Con Si or PTEN Si. Two days after siRNA transfection, the cells were immunoprecipitated with anti-Flag antibody. SA-β-Gal activity assays were conducted 4 days after siRNA transfection. The values represent the mean ± SD; n = 3; ^#P > 0.05; ***P < 0.001