Fig. 6.
The binding of mTORC1 and mTORC2 to p53 in PTEN-deficient mouse embryonic fibroblasts. a–e CrePTENfl/fl MEFs were cultured with or without 0.5 μmol/L 4-OHT for 3 days and subjected to immunoblotting (a). Relative cell number (upper graph) and Annexin V positivity (lower graph) were determined at 6 and 4 days, respectively, after 4-OHT treatment (b). Morphology and SA-β-Gal positivity were observed at 7 days after 4-OHT treatment (c). MEFs were preincubated with Rapa or Torin1 for 1 h, and then cultured with or without 0.5 μmol/L 4-OHT for 7 days. SA-β-Gal activity was determined (d). IP was performed 3 days after 4-OHT treatment (e). f A proposed signaling cascade illustrating the roles of mTORC1 and mTORC2 in directly binding and phosphorylating p53 during PICS. The values represent the mean ± SD; n = 3; #P > 0.05; **P < 0.01; ***P < 0.001