Fig. 1.

H19 promotes cell proliferation and expression of fibroid-promoting genes. a RT-qPCR results of H19 expression in fibroids and matched myometrium (control). n = 30. b Primary leiomyoma cells derived from fibroid tumors of two patients were transfected with control siRNA (siCon) or H19-specific siRNA (siH19), followed by RT-qPCR (left panels), cell viability (middle panels), and caspase-3/7 activity (right panels) analyses at 48 h post transfection. n = 3–5. c UtLM-1 or ht-UtLM cells were transfected with siCon, siH19, or a TET3-specific siRNA (siTET3). RNA levels were measured by RT-qPCR 48 h later. n = 3, one-way ANOVA with Dunnett’s post test. All data are representative of at least two independent experiments and are presented as mean ± SEM. *p < 0.05, **p < 0.01. ns, not statistically significant