Fig. 2.

H19 regulates expression of HMGA2 and TET3 posttranscriptionally via let-7. a, c UtLM cells were transfected with siCon + iCon (miRNA inhibitor control), siH19 + iCon, or siH19 + iLet7; RNA and proteins were isolated 48 h later and analyzed by RT-qPCR (left panels) or western blotting (right panels). n = 3, one-way ANOVA with Tukey’s post- test. Data are representative of two independent experiments and are presented as mean ± SEM. *p < 0.05, **p < 0.01. ns, not statistically significant. b Schematics of human and mouse TET3 mRNAs, with numbers on top depicting positions of let-7-binding sites relative to the transcriptional start sites. Figures are not drawn to scale