Fig. 2.

KDM8 is required for AR-positive prostate cancer cell proliferation and survival, and promotes androgen-independent cell proliferation and tumor growth. a Growth curves of non-malignant cell line RWPE1 and prostate cancer cell lines LNCaP, PC3, C4-2B, and C4-2B-MDVR, an enzalutamide-resistant cell line. Cells were transfected with small interfering (si)-RNAs targeting KDM8 or si-non-targeting (NT) control. Every 2 days after transfection, cell proliferation was measured by MTT assay. b Growth curves of LNCaP cells infected with KDM8 overexpressing (KDM8) or control (LKO) lentiviruses followed by maintaining in androgen-deprived media. Two days later infection, cell proliferation was measured by MTT assay at indicated times. c Tumor growth curves of KDM8 overexpressing and LKO control LNCaP cells in xenografting mouse model. Cells were injected subcutaneously into the dorsal flanks of athymic nude mice (8 mice per group) and tumor volumes were measured every week by using calipers. Tumor-bearing mice were also castrated at indicated time point. Insert: photograph of xenograft tumors. The quantitative data shown in a and b are the mean ± S.D. of three separate experiments. The average tumor volumes are presented as the mean ± S.E.M. *p < 0.05; **p < 0.01, by paired Students’ t (MTT assay) or ANOVA test (xenografting study)