Fig. 2.

Silencing efficacy of mutant-specific siRNAs on endogenous mutant p53. a−d siRNAs against R175H (a), R248W (b), R249S (c), and R273H (d) were transfected in the three cell lines with WT p53 expression and in three cell lines expressing the indicated p53 mutants, and the silencing efficacy was evaluated by immunoblotting as described above. One representative blot of at least three independent experiments is shown. Mutant p53 status of cell lines is highlighted below the blots and described in Supplementary Table 1