Fig. 6.

Relief of dominant-negative effects of mutant p53 by mutant p53-specific silencing. a−e. RKO^+/− and RKO^+/248W cells were transfected with control, pan-p53 (sh-p53) or R248W-specific shRNAs (sh-4), and analyzed as described above for efficacy of silencing (a), colony growth (b), and p53 target gene expression (c). Cell death was analyzed without (d) or with cisplatin (CDDP) treatment (e). % sub-G1 cells are indicated on the histograms (as represented by M1). Representative data are shown from three independent experiments and quantified. Bar diagrams show the mean ± standard deviation of the three independent experiments. ** indicates p value of <0.005; and ***<0.001, with n = 3 samples per group