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. 2019 Jan 11;38(18):3325–3339. doi: 10.1038/s41388-018-0673-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Wild-type BAD co-localizes strongly with mitochondria. a Z slice of confocal imaging of MDA-MB-231 cells expressing pcDNA3.2-V5-DEST vector control, WT-BAD, BAD-S118D or BAD-S118A immunostained with antibodies against BAD (green) and calnexin (endoplasmic reticulum (ER) marker) (cyan). MitoTracker™ Red and DAPI were used as mitochondria (red) and nuclei (blue) markers, respectively. Scale bar = 10 μm. b Percentage of BAD protein (BAD channel MCC fraction converted to percentage) co-localizing with mitochondria (top) and ER (bottom) (colored bars) compared to randomized control (patterned bars) (n = number of cells; Kruskal-Wallis test)