Fig. 2.

Ectopic induction rival oncogene inhibit melanoma cell growth and enhance associated phenotypes. On fifth day following rival oncogene induction with doxycycline (50–100 ng/ml), three antagonistic NRAS* + iBRAF* lines (SK-MEL-119^NRAS* and WM1361^NRAS*, GMEL^BRAF*) and two neutral BRAF* + iNRAS* (SK-MEL-63^NRAS* and A375^BRAF*) lines were assayed for their phenotypic dependence. a Senescence was detected by senescence-associated expression of β-galactosidase (SA-β-gal) staining, (b) different phases of cell cycle were detected with PI staining and c apoptosis was detected by Annexin-V staining and d colony formation was detected by 0.1% of crystal violet in 24 well plates. Student’s t test, doxycycline vs. no-doxycycline, ^◇p ≤ 0.05. Bar, 40 µm. The data presented are representative of three independent experiments