Fig. 4.

SPRY4 as a mediator of oncogene antagonism. a qPCR validating SPRY4 upregulation upon rival oncogene expression in all the four cell lines showing antagonism (SK- MEL-119^NRAS*, WM1361^NRAS*, MGH-CH-1^BRAF* and GMEL^BRAF* but b not the neutral SK-MEL-63^NRAS* and A375^BRAF* cell lines. After normalization to GUSB1 RNA, data were presented as mean ± SD of triplicates experiment. c Parallel increases in the protein level was also confirmed in two suppressive cell lines but not in two neutral cell lines after induction with/without 50–100 ng/ml of doxycycline for 2–3 days by western blotting. Student’s t test, doxycycline vs. no-doxycycline, ^◇p ≤ 0.05. The data presented are representative of three independent experiments