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. 2019 Jun 13;33(9):2227–2240. doi: 10.1038/s41375-019-0508-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Detection of immunoglobulin gene rearrangements in lymphoma specimen with low tumor load by next-generation sequencing (NGS)-based clonality assessment. B cell lymphoma specimen Blym2 (20% malignant B cells) was analyzed for IGH (IGHV-IGHD-IGHJ, IGHD-IGHJ) and IGK (IGKV-IGKVJ and IGKV/Intron-KDE) loci gene rearrangements by NGS (a–d) in two independent laboratories (Lab A and Lab B) and compared to EuroClonality/BIOMED-2 assay (e, f). The sequence of the abundant clonotypes is shown, with the nucleotide junction sequence in capitals [33], and flanking sequences in lower case, which have been trimmed to 20 nucleotides length. PCB polyclonal background