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. 2019 Jun 13;33(9):2227–2240. doi: 10.1038/s41375-019-0508-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Comparison paired diagnosis-relapse B cell lymphoma specimen by next-generation sequencing (NGS)-based clonality analysis. Two consecutive B cell lymphoma specimens (Diag1 and Relap1) from the same patient were analyzed for IGH (IGHV-IGHD-IGHJ and IGHD-IGHJ) and IGK (IGKV-IGKJ and IGKV/Intron-KDE) loci gene rearrangements by NGS (a–d) and compared to EuroClonality/BIOMED-2 assay (e, f). The sequence of the abundant clonotypes are shown, with the nucleotide junction sequence in capitals [33], and flanking sequences in lower case, which have been trimmed to 20 nucleotides length