Fig. 1.

PKNOX2 expression and promoter methylation in GC cell lines. a Infinium HumanMethylation450BeadChip revealed that PKNOX2 was preferentially methylated in GC cell lines. b PKNOX2 mRNA levels in human normal tissues, as determined by RT-PCR. c PKNOX2 mRNA expression (upper) and promoter methylation (lower) in GC cells. Methylation specific PCR (MSP) was performed to detect PKNOX2 methylation (M: methylated; U: unmethylated). d CpG island on the PKNOX2 promoter. The regions for bisulfite sequencing (BGS) and MSP are shown. Each vertical bar represents a single CpG. TSS: transcription start site. PKNOX2 is methylated in GC cell lines compared to normal gastric cell line and tissues. e PKNOX2 mRNA expression was restored upon demethylation treatment with 5-Aza