Fig. 4.

Regulation of FOXM1 and EZH2 by c-Myc, of EZH2 by FOXM1 and downregulation of EMT-related markers by targeting of c-Myc, FOXM1 and EZH2. a Expression by western blotting of c-Myc, FOXM1, EZH2, H3K27me3 and of different EMT-related markers in melanoma cell line Me79 at 72 h after transfection with two different c-Myc-specific Stealth siRNA (c-Myc siRNA-1 and siRNA-2) or with control siRNA (ctrl siRNA), or at 48 h after treatment with c-Myc inhibitor 10058-F4 at 20 μM. b, c expression by western blotting of the indicated proteins after transfection of Me79 at 72 h with two different FOXM1-specific siRNA b or with two different EZH2-specific siRNA c or with control siRNA, or at 48 h after treatment with FOXM1 inhibitor Siomycin A at 2 μM b or with EZH2 inhibitor GSK126 at 5 μM c