Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 21;38(24):4739–4754. doi: 10.1038/s41388-019-0754-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — CCT complex physically interacts with TOR components. a Co-immunoprecipitation between endogenous CCT4 and Rheb using adult extracts. Asterisk indicates heavy chain IgG. b Co-immunoprecipitation between endogenous CCT1 and Rheb using adult extracts. c MBP-pulldown assay between MBP-Rheb and GST-CCT4. Rheb^Δ49–63 and Rheb^1–48 show little binding to CCT4 compared with the full-length Rheb. Sequence comparison in the related regions of VHL and Rheb is shown in (c′). Mutated Rheb constructs are also shown. d Co-immunoprecipitation between endogenous CCT4 and S6K using adult extracts. Asterisk indicates heavy chain IgG. e Co-immunoprecipitation between endogenous CCT4 and TOR using adult extracts. f Co-immunoprecipitation between endogenous CCT4 and Rheb using S2R+ cell extracts