Fig. 6.

Tumourigenicity in vivo following reprogramming. a Subcutaneous injections of increasing numbers of parental and reprogrammed PDAC-247 cells. Parental cells formed tumours after 8–10-weeks and all mice had to be killed three months after injection (upper panel). Images of one representative tumour per group are shown. Tumour weight was measured. Data are shown as box plots with whiskers indicating the range of the data and the box representing the interquartile range (lower panel). b Haematoxylin and eosin staining and immunostaining for the differentiation marker E-CAD and PanCK for tumours derived from PDAC-247 parental cells