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. 2019 Jul 16;38(36):6382–6398. doi: 10.1038/s41388-019-0890-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Correlations between PERK and FOXO3 expression and activity in MCF-7 and MCF-7-Epi^R cells in response to epirubicin. a The epirubicin-sensitive MCF-7 and -resistant MCF-7-Epi^R cells were either left untreated or treated with 1 µM epirubicin for the times shown. Whole-cell protein lysates were then analysed by western blotting using the antibodies against the proteins indicated. Molecular weight markers are shown. The protein expression levels of P-FOXO3 (T32) (95 kDa), FOXO3 (95 kDa), FOXM1 (110 kDa) and ER stress molecules, including P-PERK (T981) (140 kDa), PERK (140 kDa), P-eIF2a (S51) (38 kDa), eIF2a (38 kDa), P-AKT (S473) (60 kDa), P-AKT (T308) (60 kDa), AKT (60 kDa), p27^Kip1 (27 kDa) and β-Tubulin (55 kDa) were investigated. b FOXO3, PERK, FOXM1, p27^Kip1 mRNA expression after treatment with epirubicin as determined by RT-qPCR. Representative RNA expression profiles of at least three independent experiments. Three technical repeats were conducted in one experiment, and the data were normalised to L19 and displayed as means ± S.E.M. The expression trends of mRNA species between MCF-7 and MCF-7-Epi^R cells are compared using two-way ANOVA (Significant ***p < 0.001, for all mRNA species, respectively)