Fig. 7.

Effects of FOXO3 ectopic expression on MCF-7-Tax^R cells in response to GSK2606414. MCF-7-Tax^R cells were transfected with empty control or FOXO3 expression vector and left untreated or treated with 2 µM GSK2606414 for 24 h. a Western blot to determine the protein expression levels for P-FOXO3 (T32) (95 kDa), FOXO3 (85 kDa) and ER stress molecules, including p-PERK (140 kDa), PERK (140 kDa), P-eIF2a (38 kDa), eIF2a (38 kDa) and β-Tubulin (55 kDa). SRB assays were carried out after 48 h and 72 h after treatment with GSK2606414 at concentrations indicated (right panels). b Transfected MCF-7-Tax^R cells were treated with 0, 0.25, 0.5, 1, 2 and 4 µM of PERK inhibitor GSK2606414 every 48 h for 15 days then stained with crystal violet in clonogenic assays. Significant *P < 0.05, **P < 0.01, ***P < 0.001