Fig. 4.

Downregulation of miR-10b and miR-222 suppressed the express of MDM2. a LN229 cells were treated with MG132 (10 μmol/L) alone for 6 h and harvested different hours after treatment as control. Cells were transfected with As-miR-10b/222 for 24 h and together with MG132 (10 μmol/L) treatment as compared. The difference levels of p53 between the two groups were analysis by WB. b Western blot analysis showed the levels of MDM2 after inhibition of miR-10b and miR-222 in LN229 and U87MG. c The level of p53 was tested by WB, after inhibition of miR-10b and miR-222 in LN229 with MDM2 stable knockdown. d Representative images of IF using anti-MDM2 antibody shows the subcellular distribution of MDM2 protein. e Western blot analysis showed the levels of MDM2 Ser166 and AKT Ser473 in LN229 cells that transfected with miRNA inhibitors as indicated. The results have been correct with the downregulation of total MDM2