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. 2018 Feb 9;24(10):1489–1501. doi: 10.1038/s41380-017-0011-3

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© The Author(s) 2018

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Fig. 2 — Behavioral and morphological effects of endogenous NMDAR1-AB of the IgG class in a mouse model with open BBB. a Demonstration of BBB leakiness in ApoE^−/− mice using an intravenously injected mixture of Evans blue (EB) and sodium fluorescein (NaFl): After brain cryopreservation/lyophilization, tracers were extracted with formamide and quantified; Student’s t-test; b Experimental outline; c Immunization: Left: GluN1 peptides (P1–P4) located in the extracellular part of the receptor were used for immunization (compare Fig. 3); middle and right: Time course of anti-ovalbumin and anti-GluN1-AB (IgG) upon immunization in ApoE^−/− and ApoE^+/+ mice; optical density at dilution 1:1000 shown; titers after day 10 reach up to 1:50,000; d Effect of MK-801 injection on activity in the open field; results presented as % change from baseline (first 4 min post MK-801 set to 100%); no difference in MK-801-induced hyperactivity between genotypes after ovalbumin immunization (one-way repeated measures ANOVA: treatment × group interaction: F_(1,17) = 0.2; p = 0.7); increase in hyperactivity (during rise, plateau, decline, and after-effect phases) upon MK-801 in ApoE^−/− but not ApoE^+/+ mice immunized against GluN1 (one-way repeated measures ANOVA: treatment × group interaction: F_(1,22) = 5.6; p = 0.028). e Quantification of Iba1⁺ and CD3⁺ cells in the hippocampus to assess inflammation in the brain; one-way ANOVA; representative pictures of Iba1 (left) and CD3 (right) stainings in the middle