Fig. 7.

Ectopic expression of USP39 and TAZ restores malignant properties of glioma cells with knockdown of USP39 in vitro and in vivo. a Western blotting analysis of lysates prepared from modified U87MG and P3 cells. GAPDH was used for normalization. b OD from CCK8 assays plotted as a function of time in hours for indicated cells to evaluate cell viability. c Graphic representation of relative luciferase activity from luciferase reporter constructs regulated by Hippo signaling in U87MG cells from indicated groups. d qRT-PCR analysis of the expression of TAZ target genes from indicated cells. GAPDH was used for normalization. e, f Representative images of luciferase bioluminescence at the indicated days after injection of U87MG and P3 cells into the brains of nude mice. Graphic representation of the total flux at the indicated days for the indicated cell types. Student’s t-test: *p < 0.05, **p < 0.01, ***p < 0.001