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. 2018 Sep 4;38(6):896–912. doi: 10.1038/s41388-018-0484-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — MYEOV transcript promotes NSCLC invasiveness and metastasis in vitro. a The protein levels of EMT markers in indicated cells examined by immunofluorescence. Scale bar: 50 μm. b The protein levels of EMT markers in indicated cells examined by WB analysis. c Invasiveness as measured by the Matrigel Transwell assay. Scale bar: 100 μm. Columns, number of cells invaded across the membrane (each bar represents the mean ± SD derived from three independent experiments, one-way ANOVA followed by Dunnett’s multiple comparison test. *P < 0.05; ns, not significant). d Representative micrographs of indicated cells grown on the Matrigel in 3-D spheroid invasion assay. Scale bar: 100 μm