Table 2. Characteristics of selected Pcal KB211 transposon disruption mutants (N = 53).
The mutated genes were determined by using Pseudomonas Genome DB BLAST search based on the genome database of Pseudomonas syringae pv. maculicola ES4326 (Pseudomonas cannabina pv. alisalensis ES4326). Functional category annotations for Pcal KB211 genes are primary based on COG (Chen et al., 2019) and KEGG (Kanehisa & Goto, 2000) annotations. Asterisks represent the mutants which virulence score was less than 1.2 on oat. The Tn5 mutants were also tested for their ability to grow on MG minimal medium. + represents positive growth. − represents negative growth.
| Classification | Mutant | Locus | Description | Virulence score | Growth in MG | ||
|---|---|---|---|---|---|---|---|
| Cabbage | Oat | ||||||
| Amino acid metabolism and transport | NF34 | PMA4326_25462 | 3-phosphoglycerate dehydrogenase | 0 | 0.29* | − | |
| NN31 | PMA4326_01907 | D-amino acid dehydrogenase small subunit | 0.29 | 0* | + | ||
| NI13 | PMA4326_20937 | N-acetyl-gamma-glutamyl-phosphate reductase | 0 | 0.14* | − | ||
| NF2 | PMA4326_02177 | Tryptophan synthase subunit alpha | 0 | 0.14* | − | ||
| NH11 | PMA4326_18563 | N-ethylammeline chlorohydrolase | 1 | 2 | + | ||
| NM37 | PMA4326_04881 | Taurine catabolism dioxygenase TauD | 0.75 | 1.67 | + | ||
| DNA processing and modification | NN6 | PMA4326_27962 | Error-prone DNA polymerase | 0.43 | 0.43* | + | |
| NV6 | PMA4326_22604 | Helicase | 1.62 | 1.5 | + | ||
| NE29 | PMA4326_20877 | Helicase UvrD | 0.33 | 2 | + | ||
| NI5 | PMA4326_29937 | Integrase | 1.2 | 2 | + | ||
| NC22 | PMA4326_30012 | Mobilization protein | 1.33 | 2 | + | ||
| NN12 | PMA4326_19710 | N-methyltransferase | 1.29 | 1.5 | + | ||
| Transcriptional regulator | NL8 | PMA4326_16681 | ArsR transcriptional factor | 0.57 | 0.71* | + | |
| NV13 | PMA4326_26857 | Fis family transcriptional factor | 1 | 0.86* | + | ||
| NK1 | PMA4326_02287 | HexR transcriptional factor | 0.43 | 0.29* | + | ||
| NN14 | PMA4326_08221 | LysR family transcriptional factor | 0.71 | 0.57* | + | ||
| NN5 | PMA4326_21784 | AraC family transcriptional regulator | 1 | 2 | + | ||
| Type IV secretion system | NK16 | PMA4326_28915 | DNA topoisomerase III | 0.29 | 0.58* | + | |
| NM29 | PMA4326_28915 | DNA topoisomerase III | 1 | 1* | + | ||
| NA13 | PMA4326_28915 | DNA topoisomerase III | 1.33 | 1.5 | + | ||
| NA4 | PMA4326_28915 | DNA topoisomerase III | 0.67 | 2 | + | ||
| NV27 | PMA4326_28955 | Relaxase | 0.63 | 1.14* | + | ||
| Nucleotide metabolism and transport | NL37 | PMA4326_26032 | D-tyrosyl-tRNA(Tyr) deacylase | 1 | 0.57* | + | |
| NF22 | PMA4326_22906 | Phosphoribosylaminoimidazole carboxylase ATPase subunit | 0.17 | 0.43* | − | ||
| NL3 | PMA4326_05636 | Polynucleotide phosphorylase/polyadenylase | 0 | 0.13* | + | ||
| NH26 | PMA4326_04134 | Dehydrogenase | 1 | 2 | + | ||
| NM30 | PMA4326_19765 | Phosphoribosyltransferase | 1.25 | 2 | + | ||
| Lipid metabolism and transport | NM2 | PMA4326_20637 | Acetyltransferase family protein | 0.71 | 0.29* | + | |
| NN3 | PMA4326_17521 | Lipid kinase | 0.57 | 0.43* | + | ||
| NM18 | PMA4326_10330 | Acyltransferase | 1 | 1.67 | + | ||
| Carbohydrate metabolism and transport | NM10 | PMA4326_26042 | Glycogen phosphorylase | 0.57 | 1* | + | |
| NN1 | PMA4326_09615 | Glycerol kinase | 1.5 | 1.67 | + | ||
| Energy generation | NU21 | PMA4326_20235 | Malate:quinone oxidoreductase | 0.38 | 0.29* | + | |
| NV7 | PMA4326_01045 | Polyphosphate kinase | 0.83 | 0.86* | + | ||
| Membrane transport | NU19 | PMA4326_12408 | RND transporter | 0.75 | 1* | + | |
| NU37 | PMA4326_26242 | Sulfonate ABC transporter substrate-binding protein | 0.25 | 1.14* | + | ||
| Peptidoglycan/cell wall polymers | NC15 | PMA4326_15504 | D-alanyl-D-alanine carboxypeptidase | 0 | 1.14* | + | |
| NN13 | PMA4326_23061 | N-acetylmuramoyl-L-alanine amidase | 0.57 | 1.14* | + | ||
| Signal transduction mechanisms | NM38 | PMA4326_16771 | Methyl-accepting chemotaxis protein | 0.57 | 0.57* | + | |
| NH7 | PMA4326_04474 | HDOD domain protein | 0.33 | 2 | + | ||
| Type III secretion system | NB35 | PMA4326_02782 | Type III secretion protein HrcQb | 0 | 0.5* | + | |
| Cofactor metabolism | NC12 | PMA4326_20982 | Lipoate-protein ligase | 0.83 | 1.14* | + | |
| Nitrogen metabolism | NU13 | PMA4326_16286 | Nitrate reductase | 0.5 | 1.14* | + | |
| Stress resistance | NU25 | PMA4326_00370 | 16S rRNA methyltransferase | 0.13 | 0.71* | + | |
| Iron metabolism and transport | NI6 | PMA4326_03299 | Heme oxygenase | 1.4 | 2 | + | |
| Hypothetical | NN20 | PMA4326_19408 | Hypothetical protein | 0.29 | 0.43* | + | |
| NV25 | PMA4326_10750 | YD repeat-containing protein | 1 | 0.57* | + | ||
| NI7 | PMA4326_30377 | Hypothetical protein | 1.63 | 1.25 | + | ||
| NL30 | PMA4326_27167 | Hypothetical protein | 1.25 | 1.67 | + | ||
| NM27 | PMA4326_29225 | Hypothetical protein | 0.25 | 1.67 | + | ||
| NM32 | PMA4326_27567 | Hypothetical protein | 1 | 2 | + | ||
| NV33 | PMA4326_08475 | Hypothetical protein | 1.2 | 2 | + | ||
| NK39 | PMA4326_18613 | Rhodanese-like domain protein | 0 | 1.67 | + | ||