Table 1. Assembly comparison of Asclepias, Calotropis, Catharanthus, Rhazya, and Coffea.

Species	Genome size (Mbp)	Assembly size (Mbp)	N50 (kbp)	# Scaffolds	Sequencing method
Coffea canephora	710	568.6	1,261	13,345	454 SE & mate-pair, Illumina SE & PE, BACs, haploid accession
Rhazya stricta	200	274	5,500	980	Illumina PE & mate-pair, PacBio, optical mapping
Catharanthus roseus	738	506	27.3	41,176	Illumina PE, inbred accession
Calotropis gigantea	225	157.3	805	1,536	Illumina PE & mate-pair
Asclepias syriaca	420	156.6	3.4	54,266	Illumina PE & mate-pair

Notes:

Sequencing method includes technologies and materials used in sequencing.

N50, 50% of the assembly is contained in scaffolds of this length or larger; BAC, bacterial artificial chromosome; SE, single-end; PE, paired-end.