Figure 4.

Effects of muscone on migration and wound healing capabilities. (A) GMSCs were seeded in the upper chamber. After 24 hrs of incubation with (b, c, d) or without muscone (a), the cells that migrated to the lower surface of the membrane were stained. GMSC monolayers were scratched and incubated with (f, g, h) or without muscone (e) for 24 hrs. Scale bar: 200 μm. a, e: 0 mg/L muscone; b, f: 3 mg/L muscone; c, g: 6 mg/L muscone; d, h: 9 mg/L muscone. (B) The number of cells that migrated to the lower surface of the membrane was calculated. (C) The migration area was analyzed using ImageJ software. Data are presented as the mean ± S.E.M. of three independent experiments. **P<0.01, ***P<0.001, compared with the control group (0 mg/L muscone).

Abbreviations: GMSCs, gingival mesenchymal stem cells; S.E.M., standard error of the mean.