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. 2019 Sep 18;13:3291–3306. doi: 10.2147/DDDT.S220970

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© 2019 Yuan et al.

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Effect of muscone on adipogenic differentiation. (A) GMSCs were cultured in adipogenesis-inducing medium for 21 days, and oil red O staining was performed. a: 0 mg/L muscone; b: 3 mg/L muscone; c: 6 mg/L muscone; d: 9 mg/L muscone. Scale bar: 50 μm. (B) The relative number of lipid droplets was quantified based on the absorbance at 510 nm. (C, D) GMSCs were cultured in adipogenesis-inducing medium for 3 and 6 days. The expression of the adipogenesis-related genes PPARγ and LPL was measured by RT-PCR. Data are presented as the mean ± S.E.M. of three independent experiments. *P<0.05, **P<0.01, ***P<0.001, compared with the control group (0 mg/L muscone).

Abbreviations: GMSCs, gingival mesenchymal stem cells; S.E.M., standard error of the mean; RT-PCR, real-time polymerase chain reaction.