Fig. 4.

Arid1a knockout impairs lymphoid development. a Representative FACS plots show maturational stages of B cells in the BM of Arid1a^f/f;Vav-iCre⁺ and control mice. b, c Proportion of cells in different B-cell developmental stages in the BM of WT and Arid1a KO [Vav-iCre (b) (n = 5) and Mx1-Cre (c) (n = 6)] mice. proB: CD43⁺B220⁺, preB: CD43⁻B220⁺IgM⁻, immature B (Imm. B): CD43⁻B220⁺IgM⁺, mature B (Mat. B): CD43⁻B220^hiIgM⁺, Fraction A (Fr. A): CD24⁻BP1⁻ proB cells, Fraction B (Fr. B): CD24⁺BP1⁻ proB cells, Fraction C (Fr. C): CD24⁺BP1⁺ proB cells. d Thymus cellularity in Arid1a^f/f;Vav-iCre⁺ and control (either Arid1a^f/f;Vav-iCre⁻ or Arid1a^f/+;Vav-iCre⁻ littermates) mice (11–20 weeks). e FACS plots depict representative staining of four major thymic populations: DN (CD4⁻CD8⁻), DP (CD4⁺CD8⁺), CD4⁺ and CD8⁺ SP cells in the thymus of Arid1a^f/f;Vav-iCre⁺ and control mice. f Frequencies of DP, DN, CD4⁺ SP and CD8⁺ SP populations in Arid1a^f/f;Vav-iCre⁺ and control mice (n = 5). g Representative flow cytometry staining for CD44 and CD25 expression within the DN population in the thymus of Arid1a KO (Vav-iCre) and control mice. h Proportions of DN1 (CD44⁺CD25⁻ DN), DN2 (CD44⁺CD25⁺ DN), DN3 (CD44⁻CD25⁺ DN) and DN4 (CD44⁻CD25⁻ DN) sub-populations within the DN compartment of thymus of Arid1a^f/f;Vav-iCre⁺ and control mice (n = 5). i Frequencies of B cells (CD19⁺) and T cells (CD3⁺) in the spleen of WT and Arid1a KO mice (n = 5). Data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns = not significant