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. 2018 Oct 23;38(11):1787–1801. doi: 10.1038/s41388-018-0551-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Stat6 regulates IEC death and alkylating-inducing DNA damage a–l. a–c Immunohistochemical staining and index of cleaved CASPASE 3 expression in unchallenged (d0) and AOM-treated (8 h) WT and Stat6^−/− IEC (WT d0 n = 6; Stat6^−/− d0 n = 3; WT 8 h AOM n = 8; Stat6^−/− 8 h AOM n = 6); d–f Immunohistochemical staining and index of cleaved CASPASE 3 expression in IEC on day 8 (d 8) of the CAC model (n = 5/genotype); g, h Immunohistochemical staining and index of p-H2AX in AOM-treated (4 h) WT and Stat6^−/− IEC (WT 4 h AOM n = 7; Stat6^−/− 4 h AOM n = 6); i Immunoblot analysis of H2AX phosphorylation and STAT6 in WT (Stat6^+/+) and Stat6^−/− colonic organoids treated 2.5 h with 25 μM MNNG or 0.03% DMSO in PBS (sham). β-actin as loading control; j Immunoblot analysis of p-ATM, p-CHK2, IRE1-a, p-EIF2A, p-PERK, CHOP, BCL-XL, BCL-2, BAX, and STAT6 performed with IEC lysates from unchallenged (d0) or AOM-treated (4 h) mice. β-actin as loading control; k, l Immunohistochemical staining and index of p-P53 S15 expression in unchallenged (d0) and AOM-treated (8 h) WT and Stat6^−/− IEC (WT d0 n = 6; Stat6^−/− d0 n = 3; WT 8 h AOM n = 9; Stat6^−/− 8 h AOM n = 6). Data are mean ± SEM