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. 2019 Jun 26;160(10):2305–2315. doi: 10.1097/j.pain.0000000000001620

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Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Figure 5. — NGF^R121W-SNAP-guided photoablation induces reversible nerve retraction without affecting keratinocytes in the skin. (A–D) Representative paw skin cryosections stained for keratin 14 (K14, in green) and TUNEL TMR (in red) with quantitation of keratinocytes cell death in mice (n = 3 animals) injected for 3 days with IR700 followed by NIR illumination (A), NGF^R121W-SNAP+IR700 followed by NIR illumination (B and C). Skin was collected 7 days after (A and B) and 28 days after (C) the last day of injection. The number of TUNEL⁺ keratinocytes is expressed as percentage (D). (E–H) Representative paw skin cryosections stained for the neuronal marker Pgp9.5 (in green) of mice injected for 3 days with IR700 followed by NIR illumination (E), NGF^R121W-SNAP+IR700 followed by NIR illumination (F and G). The skin was collected 7 days after (E and F) and 28 days after (G) the last day of injection. Arrows indicate free nerve endings. Quantitation of the epidermal free nerve endings for the indicated sample; 8 sections were analyzed (H). *P = 0.001 (two-tailed t test). NIR, near-infrared.